Return to the Orchids Forum | Post a Follow-Up

 o
Virus Self Testing

Posted by caromo 8.5 SC (My Page) on
Thu, Sep 17, 09 at 19:40

Just curious what your experiences have been with the virus self-testing kits / strips. In reading some other posts, it sounds as if many of you do follow-up send out testing -- what do you find with the reliability of results in self vs. send out? Also, in researching the Agdia site, there was reference to not only the test strips but a buffer as well -- are these the only two things you need or is there more?

Thanks for any insight you can share


Follow-Up Postings:

 o
RE: Virus Self Testing

I've used hundreds of these test kits without any problems. I used to send the positive results out for confirmation by ELISA, but I quit doing it because the results were always the same. There are more accurate tests available, but these seem to work quite well.

The buffer solution is already inside the bag with the abrasive grid. If you had lots of samples to test and they were already ground up, you could just add buffer and insert the test strip. I always use the test kits. Once you get the hang of it, you can do composite testing with 2 or 3 leaf sections from different plants combined to make a single sample. This is how I test flasks and compots. If I really like a particular flower, I perform individual virus testing before it becomes part of my core collection, even if it tested negative as part of a composite test. I've never had contradictory results, so this may be redundant, but I do it anyway.

If you decide to begin testing your plants and you have a lot of phals, be prepared for some bad news.

Mike


 o
RE: Virus Self Testing

Thanks so much for the info -- very helpful!


 o
RE: Virus Self Testing

Mike wrote, "I used to send the positive results out for confirmation by ELISA, .....the results were always the same."

That has been my experience to date. For really valued individual plants, I may choose to do that again.

--Stitz--


 o
RE: Virus Self Testing

Glad to hear that self-testing is pretty reliable.

One other question -- in your experience(s), are there some orchid species / hybrids which seem less virus-prone than others, or are they all fair game? Seems like phals are highly susceptible, and those are the ones in my collection I'm most concerned about (appreciate your comment, Mike!) -- a couple of catts I want to check, and an oberonia which looks iffy. Understand they can be totally asymptomatic, but wonder if there are some that just have higher susceptibility?


 o
RE: Virus Self Testing

Occasionally, Andy Easton mentions this topic on another forum. Perhaps, you can find more through his name?


 o Post a Follow-Up

Please Note: Only registered members are able to post messages to this forum.

    If you are a member, please log in.

    If you aren't yet a member, join now!


Return to the Orchids Forum

Information about Posting

  • You must be logged in to post a message. Once you are logged in, a posting window will appear at the bottom of the messages. If you are not a member, please register for an account.
  • Posting is a two-step process. Once you have composed your message, you will be taken to the preview page. You will then have a chance to review your post, make changes and upload photos.
  • After posting your message, you may need to refresh the forum page in order to see it.
  • Before posting copyrighted material, please read about Copyright and Fair Use.
  • We have a strict no-advertising policy!
  • If you would like to practice posting or uploading photos, please visit our Test forum.
  • If you need assistance, please Contact Us and we will be happy to help.


Learn more about in-text links on this page here