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Getting rid of deadly nightshade

I created a new bed under a newly trimmed-evergreen for some small hostas and heucheras. Under the juniper tree before we trimmed the bottom 2 feet of scruffy branches out, we were always pulling out weeds, mostly deadly night shade (purple-black berries, tomato-looking flowers). I broke up all the clods carefully to remove parts of weeds, but sure enough, the bed has only been planted a week or so and more night shade is popping up. Assuming that pulling them is not enough, what about painting them with Round-up? Will that work its way down the roots and kill them?

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