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technical information about Rosa rugosa leaf distortion virus

Posted by henry_kuska z5 OH (kuska@neo.rr.com) on
Tue, Nov 26, 13 at 13:15

Published in: Archives of Virology, December 2013, Volume 158, Issue 12, pp 2617-2620

Title: "Complete nucleotide sequence of Rosa rugosa leaf distortion virus, a new member of the family Tombusviridae"
Authors: Dimitre Mollov, Ben Lockhart, David C. Zlesak

"Abstract
This report describes the complete nucleotide sequence and genome organization of Rosa rugosa leaf distortion virus (RrLDV), the causal agent of a previously undescribed virus disease of Rosa rugosa. The RrLDV genome is a positive-sense ssRNA, 3971 nucleotides in length, containing five open reading frames (ORFs). ORF1 encodes a 27-kDa peptide (p27). ORF2 shares a common start codon with ORF1 and continues through the amber stop codon of p27 to produce an 87-kDa protein (p87) with amino acid sequence similarity to the RNA-dependent RNA polymerases (RdRp) of members of the family Tombusviridae. ORF3 encodes a protein of 8 kDa with no significant similarity to known viral sequences. ORF4 encodes a 6-kDa protein (p6) with similarity to the p13 movement proteins of members of the family Tombusviridae. ORF5 has no conventional start codon and overlaps with p6. A putative +1 frame shift mechanism allows p6 translation to continue through the stop codon and results in a 12-kDa protein with high homology to the carmovirus p13 movement protein. The 37-kDa protein encoded by ORF6 has amino acid sequence similarity to coat proteins (CPs) of members of the family Tombusviridae. Phylogenetic analyses of the RdRp and CP amino acid sequences placed RrLDV in a subgroup close to members of the genus Carmovirus of the family Tombusviridae."

See:
http://link.springer.com/article/10.1007/s00705-013-1763-y?no-access=true

Here is a link that might be useful: link for above


Follow-Up Postings:

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RE: technical information about Rosa rugosa leaf distortion virus

Henry,
Any mention in the article about what part of the world the sick plants exist in? or what the symptoms might be?
It's going to be a while before I get to the Ag library.

Ann


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RE: technical information about Rosa rugosa leaf distortion virus

"The virus used in this study was obtained from naturally
infected plants of Rosa rugosa ‘Charles Albanel’."

This is all that I could find in the full paper. It does refer to a 2011 paper:

8. Lockhart B, Zlesak D, Fetzer J (2011) Identification and partial characterization of six new viruses of cultivated roses in the USA.
Acta Hortic 901:139��"147
Since the 2011 paper has Jodi Fetzer's of the New York Botanical Garden name as one of the authors, perhaps the sample came from there. Please notice that this is only a "PERHAPS".

"Recently, we described a virus with spherical particles [8] associated with one of several new foliar diseases of rose. The virus was named Rosa rugosa leaf distortion virus (RrLDV) after the typical symptoms associated with infection by this virus. Both the disease and the virus were graft transmitted from diseased to healthy R. rugosa, suggesting that RrLDV is likely the causal agent of the disease (our unpublished results). The objectives of this study were to characterize the RrLDV genome and
determine its taxonomic and phylogenetic relationship to
known viruses."
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H.Kuska comment; I am frustrated that so many of these individual papers offer so little information of usefullness to the average rose grower. Possibly the editors pressure the authors to be as consise as possible.


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RE: technical information about Rosa rugosa leaf distortion virus

Dave Zlesak has commented on a future publication that appears to be more consumer orientated.

http://www.rosebreeders.org/forum/viewtopic.php?f=2&t=54298

Here is a link that might be useful: link for above


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